Advanced Live Cell Imaging System

The SLV-40i is designed to meet the rigorous demands of mammalian cell culture. It utilizes an LWD Infinity Plan optical system that provides exceptional clarity through the thick bottoms of culture vessels. As a dedicated inverted phase contrast microscope, it allows for high-contrast cell morphology observation of unstained, transparent specimens, making it indispensable for cell line development.

The system's long working distance microscope condenser (N.A. 0.3, WD 72mm) can be detached to increase working distance up to 195mm, accommodating large culture flask microscopy and roller bottles.

Versatile Culture Vessel Handling

Efficiency in drug screening and cell-based assays relies on handling various vessel formats. The SLV-40i features a large mechanical stage (210 x 241 mm) equipped with a suite of holders for:

• 96-Well Plate Microscope applications for high-throughput screening.
• Petri Dish Microscope observation (35mm and 65mm dishes).
• Terasaki Holders for micro-volume applications.
• Standard Glass Slides for primary cell culture imaging.

Illumination & Digital Documentation

The microscope is powered by a 5W LED illumination system (Transmit & Reflect capable). The cool LED light prevents thermal damage to sensitive samples during long-term time-lapse imaging. Uniquely, the system supports a 5V 1A output, allowing it to run on a power bank, ensuring continuous organoid microscopy even during power interruptions.

The Seidentopf Trinocular Head includes a light split switch (E100:P0 / E20:P80), enabling simultaneous observation and digital microscopy documentation when paired with a camera.

Specification	Details
Optical System	Infinity Plan Phase Contrast System
Objectives	LWD Plan Phase: 4x, 10x, 20x, 40x
Stage	210 x 241 mm (Moving Range 128 x 80 mm)
Condenser	LWD N.A. 0.3 (WD 72mm / 195mm detached)
Holders	96-Well, Terasaki, Petri Dish (35/65mm), Slide
Focusing	Coaxial Coarse/Fine (2 µm precision)
Illumination	5W LED (Transmit) + Optional Fluorescence

Frequently Asked Questions

1. What is the advantage of an inverted microscope?

An inverted microscope places the objectives below the stage, allowing you to view samples in large containers like flasks and petri dishes. This is essential for adherent cell imaging where cells grow on the bottom of the vessel.

2. Does it support Phase Contrast?

Yes, the SLV-40i is a fully capable inverted phase contrast microscope. It comes with Phase Contrast objectives and sliders, enabling clear visualization of transparent, unstained live cells.

3. Can I use 96-well plates with this model?

Absolutely. The mechanical stage includes a specific holder for multi-well plate imaging, making it perfect for cytotoxicity assessment and high-throughput drug screening.

4. Is it suitable for fluorescence microscopy?

Yes, the SLV-40i is upgradeable. It can be configured as an inverted fluorescence microscope by adding the Epi-Fluorescent attachment, suitable for cell transfection microscopy and GFP imaging.

5. What is the "Long Working Distance" (LWD)?

Long working distance means the condenser and objectives are designed to focus through thick plastic or glass. The condenser has a WD of 72mm, which can be extended to 195mm for tall culture flasks.

6. Can I attach a camera?

Yes. The trinocular head features a dedicated camera port with a light split optical path, allowing you to capture images for cell viability assessment reports while simultaneously viewing through the eyepieces.

7. Does it heat up the sample?

No. The system uses 5W LED illumination, which emits very little heat compared to halogen bulbs. This protects your samples during live cell imaging sessions.

8. Can I use it inside a biosafety cabinet?

Yes, the compact and ergonomic design makes it suitable for use inside laminar flow hoods for sterile stem cell microscopy and primary cell culture work.