Reliable AutoGrid Assembly

The AutoGrid preparation process is a critical step in the TFS autoloader clipping workflow. Our C-clip insertion tool features a precision-machined clipping tool barrel that guides the C-clip directly into the AutoGrid ring groove. This compact clipping tool design minimizes user error and significantly improves the AutoGrid clipping success rate.

Designed for use in a cryogenic environment, the tool acts as a C-clip seating tool, ensuring the clip is fully engaged. This prevents "mis-bottoming"—a common issue where the clip pops out during Krios grid clipping or cassette loading.

High-Throughput Clipping Workflow

For labs performing batch grid clipping, having multiple pre-loaded tools is essential. Using a clipping tool rack allows users to prepare several C-clips in advance, streamlining the cryogenic clipping workflow. The tools are designed to be frost-free when kept under LN2 vapor, reducing ice contamination prevention concerns during the sensitive assembly phase.

Key Features

• Dual-Ended Design: Often features a loading end and a seating end for efficient AutoGrid ring assembly.
• Cylinder Guide: The clipping tool cylinder guide ensures perfect vertical alignment, preventing bent clips.
• Ergonomic Handling: Can be operated easily with tweezer-operated clipping tool techniques or manually with gloved hands.
• Durability: Constructed from materials that withstand repeated thermal cycling during multiple round clipping sessions.

Frequently Asked Questions

1. Does this tool work with standard Thermo Fisher AutoGrids?

Yes. The tool is engineered to compatible tolerances for all standard AutoGrid clipping accessories used in Titan Krios, Glacios, and Talos systems.

2. What is "C-clip bottoming"?

C-clip bottoming refers to pushing the clip all the way down into the ring's groove. If a clip is not fully bottomed, it can jam the microscope's autoloader. This tool is specifically designed to apply even pressure to fully seat the clip.

3. Can I use this under Liquid Nitrogen?

Absolutely. The tool is designed for grid clipping under liquid nitrogen. Its thermal mass and material properties are optimized to function reliably at cryogenic temperatures without freezing up.

4. Do I need a clipping station to use this?

While a full clipping station provides stability, this C-clip loading tool is the handheld component used within the station to physically insert the clip. It is an essential part of the AutoGrid assembly tools kit.

5. How do I prevent ice contamination?

By using a dedicated, pre-cooled insertion tool, you reduce the time the grid is exposed to ambient humidity. The tool's efficient design speeds up the process, which is the best method for ice contamination prevention.