The LUNA-FL™ Dual Fluorescence Cell Counter is an advanced, high-performance automated cell counting device designed for precise cell viability, concentration, and fluorescence-based analysis. Featuring brightfield and dual fluorescence (green/red) detection, it enables accurate live/dead cell discrimination using Acridine Orange/Propidium Iodide (AO/PI) staining.
This powerful instrument is ideal for mammalian cells, yeast, primary cells, and transfected cells, making it a versatile solution for research, cell culture monitoring, and biopharmaceutical applications.
1. Dual Fluorescence and Brightfield Cell Counting
- Supports fluorescence (AO/PI staining) and brightfield (Trypan Blue) counting.
- Eliminates debris and dead cell interference, ensuring highly accurate viability assessment.
2. Automated and Precise Cell Analysis
- Advanced detection algorithms provide reproducible cell concentration and viability results.
- Automated autofocusing and cell size gating for reliable measurements.
- 3. Fast and High-Throughput Operation
- Rapid cell counting in less than 30 seconds.
- Compatible with disposable counting slides for easy, contamination-free analysis.
4. GFP Transfection Efficiency Analysis
- Enables fluorescence-based cell transfection efficiency measurement for GFP-expressing cells.
- Quantifies transfected vs. non-transfected cell populations with high sensitivity.
5. Wide Application Range
- Ideal for mammalian, yeast, and stem cell research.
- Supports cell-based assays, bioprocess monitoring, and drug discovery.
6. Compact, User-Friendly Design
- Standalone system with an intuitive touchscreen interface.
- No calibration required, ensuring plug-and-play convenience.
| Specification | Details |
|---|---|
| Counting Methods | Brightfield and Dual Fluorescence (AO/PI, Trypan Blue) |
| Cell Size Range | 1 – 60 µm |
| Counting Time | Less than 30 seconds |
| Viability Staining | Acridine Orange/Propidium Iodide (AO/PI), Trypan Blue, Erythrosin B |
| Transfection Efficiency | GFP Fluorescence Quantification |
| Focusing | Automated |
| Display | 7-inch Touchscreen |
| Data Storage | Up to 1,000 Counts |
| Connectivity | USB Export |
| Power Supply | 110V/220V, 50/60Hz |
| Dimensions | Compact Benchtop Design |
| Weight | 2.3 kg |
| Warranty | 1-2 Years |
1. Cell Viability and Cytotoxicity Analysis
- AO/PI and Trypan Blue staining for high-accuracy viability assessment.
- Eliminates counting errors due to debris or cell clusters.
2. GFP Transfection Efficiency Measurement
- Quantifies GFP-expressing cells for gene expression and transfection studies.
- Ensures precise transfection efficiency analysis in fluorescence mode.
3. Yeast Cell Counting and Viability Assessment
- Optimized for yeast culture monitoring with fluorescence staining.
- Enables high-throughput analysis in fermentation and bioprocessing.
4. Cell-Based Assays in Biopharma and Research
- Supports stem cell research, cancer biology, immunology, and drug discovery.
- Compatible with cell proliferation and toxicity screening assays.
